Benzo C A, Stearns S B
Am J Anat. 1976 Nov;147(3):273-9. doi: 10.1002/aja.1001470303.
A radioimmunoassay for glucagon, together with electron microscopic observations of early embryonic alpha cells were utilized to examine developmental aspects of glucagon accumulation and release in the chick embryo. Immunoreactive glucagon was detected in both the pancreas and blood plasma from the fifth embryonic day onwards. In addition, emiocytotic events were observed in alpha cells as early as the fifth embryonic day. The early appearance of glucagon and its subsequent developmental profile correlate well with major events in carbohydrate metabolism occurring in the embryonic chick, and are discussed in relation to a functionally responding system, the developing liver. The present data show that glucagon is secreted at earlier embryonic stages than hitherto demonstrated, and suggest a developmental role for glucagon in hepatic glycogen metabolism.
利用一种胰高血糖素放射免疫测定法，结合对早期胚胎α细胞的电子显微镜观察，来研究鸡胚中胰高血糖素积累和释放的发育情况。从胚胎第5天起，在胰腺和血浆中均检测到免疫反应性胰高血糖素。此外，早在胚胎第5天就在α细胞中观察到了胞吐事件。胰高血糖素的早期出现及其随后的发育情况与胚胎期鸡碳水化合物代谢中的主要事件密切相关，并结合一个功能反应系统——发育中的肝脏进行了讨论。目前的数据表明，胰高血糖素在胚胎发育早期就开始分泌，这比迄今所证明的时间更早，并提示胰高血糖素在肝脏糖原代谢中具有发育作用。
