Peluso G, Porta R, Esposito C, Tufano M A, Toraldo R, Vuotto M L, Ravagnan G, Metafora S
Institute of Protein Biochemistry and Enzymology, National Research Council, Arco Felice, Naples, Italy.
Biol Reprod. 1994 Mar;50(3):593-602. doi: 10.1095/biolreprod50.3.593.
The pretreatment of epididymal spermatozoa with SV-IV, one of the major secretory protein produced by the epithelium of adult rat seminal vesicles, was found to markedly decrease their ability to induce in vivo peritoneal macrophage activation, measured as class II major histocompatibility complex surface antigen expression, superoxide anion production, phagocytic activity, and antigen presentation. In addition, the treatment of spermatozoa with SV-IV produced a significant decrease of their immunogenicity evaluated in vitro by [3H]thymidine incorporation in splenocyte/spermatozoon co-culture. The concurrent presence of SV-IV and transglutaminase, an enzyme secreted in large amounts from the rat anterior prostate, amplified these phenomena. The suppression of the epididymal sperm immunogenicity is suggested to be of crucial importance for the prevention of the immune response to the sperm introduced in the immunocompetent female genital tract during coitus.
成年大鼠精囊上皮产生的主要分泌蛋白之一SV-IV对附睾精子进行预处理后，发现其诱导体内腹膜巨噬细胞活化的能力显著降低，这一能力通过II类主要组织相容性复合体表面抗原表达、超氧阴离子产生、吞噬活性和抗原呈递来衡量。此外，用SV-IV处理精子后，通过脾细胞/精子共培养中[3H]胸腺嘧啶核苷掺入法在体外评估发现其免疫原性显著降低。SV-IV与转谷氨酰胺酶（一种从大鼠前列腺前部大量分泌的酶）同时存在会放大这些现象。附睾精子免疫原性的抑制对于预防交配期间引入免疫活性雌性生殖道中的精子的免疫反应至关重要。
