Petrov V, Amery A, Lijnen P
Department of Molecular and Cardiovascular Research, University of Leuven, Belgium.
Eur J Biochem. 1994 Apr 1;221(1):195-9. doi: 10.1111/j.1432-1033.1994.tb18729.x.
Human atrial natriuretic peptide (ANP) fragments ANP-(127-150) or ANP-III and ANP-(127-149) or ANP-II activate Na+/H+ exchange in human erythrocytes at concentrations as low as 1 pM. Both ANP-(127-147) or ANP-I and ANP-(129-150) or des-Ser5, Ser6-ANP-III have no effect on erythrocyte Na+/H+ exchange. ANP-III also produces a time-dependent increase of intraerythrocyte guanosine 3',5'-phosphate (cGMP) concentration. M&B 22,948, a specific inhibitor of cGMP phosphodiesterase, increases Na+/H+ exchange and the intracellular concentration of cGMP. Both 8-bromoguanosine 3',5'-phosphate (8-Br-cGMP) and dibutyryl-cGMP mimic the effect of ANP-III on erythrocyte Na+/H+ exchange. Our data suggest that human erythrocytes possess guanylate-cyclase activity stimulated by ANP-III and that activation of Na+/H+ exchange by this peptide is mediated by cGMP. Human erythrocytes display a high degree of sensitivity to ANP-III or ANP-II and a specificity for ANP-fragment structures just as cells with established ANP-specific receptors.
人心房利钠肽（ANP）片段ANP-(127 - 150)或ANP-III以及ANP-(127 - 149)或ANP-II，在低至1皮摩尔的浓度下就能激活人红细胞中的Na⁺/H⁺交换。ANP-(127 - 147)或ANP-I以及ANP-(129 - 150)或去-Ser⁵，Ser⁶-ANP-III对红细胞Na⁺/H⁺交换没有影响。ANP-III还会使红细胞内鸟苷3'，5'-磷酸（cGMP）浓度随时间增加。cGMP磷酸二酯酶的特异性抑制剂M&B 22,948可增加Na⁺/H⁺交换以及cGMP的细胞内浓度。8-溴鸟苷3'，5'-磷酸（8-Br-cGMP）和二丁酰-cGMP都能模拟ANP-III对红细胞Na⁺/H⁺交换的作用。我们的数据表明，人红细胞具有受ANP-III刺激的鸟苷酸环化酶活性，并且该肽对Na⁺/H⁺交换的激活是由cGMP介导的。人红细胞对ANP-III或ANP-II表现出高度敏感性，并且对ANP片段结构具有特异性，就如同具有已确定的ANP特异性受体的细胞一样。
