Petrov V, Lijnen P
Department of Molecular, University of Leuven (Katholieke Universiteit Leuven, Belgium.
Am J Physiol. 1996 Nov;271(5 Pt 1):C1556-64. doi: 10.1152/ajpcell.1996.271.5.C1556.
Guanylate cyclase activity in human erythrocytes is investigated by evaluating the intracellular guanosine 3',5'-cyclic monophosphate (cGMP) content in the presence of various agents that exert specific effects on soluble or particulate guanylate cyclase. The increase in the intraerythrocyte cGMP content by the soluble guanylate cyclase activators nitroprusside and NaNO2 suggests the presence of this enzyme in human erythrocytes. The effects of four different atrial natriuretic peptide (ANP) fragments on the intraerythrocyte cGMP content is also studied. ANP II and ANP III increase the intraerythrocyte cGMP content, whereas ANP I and des-Ser5,des-Ser6-ANP III are ineffective. Thus our data show that human erythrocytes possess particulate guanylate cyclase together with the soluble enzyme. The ANP fragments ANP II and ANP III also activate the erythrocyte Na+/H+ exchange. Nitroprusside, M & B 22948 (an inhibitor of cGMP phosphodiesterase), and the cGMP analogues dibutyryl cGMP and 8-bromoguanosine 3',5'-cyclic monophosphate also increase the erythrocyte Na+/H+ exchange rate. The latter data also suggest that the erythrocyte Na+/H+ exchange is regulated by cGMP.
通过评估在对可溶性或颗粒性鸟苷酸环化酶有特定作用的各种试剂存在下细胞内鸟苷 3',5'-环磷酸（cGMP）的含量，来研究人红细胞中的鸟苷酸环化酶活性。可溶性鸟苷酸环化酶激活剂硝普钠和亚硝酸钠使红细胞内 cGMP 含量增加，这表明人红细胞中存在这种酶。还研究了四种不同的心房利钠肽（ANP）片段对红细胞内 cGMP 含量的影响。ANP II 和 ANP III 增加红细胞内 cGMP 含量，而 ANP I 和去 - Ser5，去 - Ser6 - ANP III 则无效。因此，我们的数据表明人红细胞除了含有可溶性酶外还拥有颗粒性鸟苷酸环化酶。ANP 片段 ANP II 和 ANP III 也激活红细胞的 Na⁺/H⁺交换。硝普钠、M & B 22948（一种 cGMP 磷酸二酯酶抑制剂）以及 cGMP 类似物二丁酰 cGMP 和 8 - 溴鸟苷 3',5'-环磷酸也增加红细胞的 Na⁺/H⁺交换速率。后一组数据也表明红细胞的 Na⁺/H⁺交换受 cGMP 调节。
