星形胶质细胞对5-羟色胺能纤维变性的长期反应。

Long-term astroglial reaction to serotonergic fiber degeneration.

作者信息

Fages C, Le Prince G, Didier-Bazes M, Rolland B, Hardin H, Tardy M

机构信息

INSERM U282, Hôpital Henri Mondor, Creteil, France.

出版信息

Brain Res. 1994 Mar 7;639(1):161-6. doi: 10.1016/0006-8993(94)91777-9.

DOI:10.1016/0006-8993(94)91777-9

PMID:7910108

Abstract

Glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS) expression were analysed by Western and Northern blotting in the hippocampus, the frontal and occipital cortex, and the cerebellum of the adult rat, as a manifestation of the astroglial reaction, 2 and 3 months after 5,7-dihydroxytryptamine injection into the lateral ventricule. 5HT injury stimulated GFAP and GS expression in a temporally and regionally specific fashion. At 2 months postlesion, the GFAP-mRNA and GFAP levels appeared enhanced but returned to control levels at 3 months. The GFAP-mRNA and GS-mRNA levels increased in the frontal cortex at 3 months. Such a delayed astroglial reactivity might implicate astrocytes in neurodegenerative disorders.

摘要

通过蛋白质免疫印迹法和Northern印迹法分析成年大鼠海马体、额叶和枕叶皮质以及小脑中的胶质纤维酸性蛋白（GFAP）和谷氨酰胺合成酶（GS）表达，以此作为向侧脑室注射5,7-二羟色胺后2个月和3个月时星形胶质细胞反应的表现。5-羟色胺损伤以时间和区域特异性方式刺激GFAP和GS表达。损伤后2个月，GFAP-mRNA和GFAP水平似乎升高，但在3个月时恢复到对照水平。3个月时额叶皮质中的GFAP-mRNA和GS-mRNA水平升高。这种延迟的星形胶质细胞反应性可能与神经退行性疾病中的星形胶质细胞有关。