Shah N M, Marchionni M A, Isaacs I, Stroobant P, Anderson D J
Division of Biology, California Institute of Technology, Pasadena 91125.
Cell. 1994 May 6;77(3):349-60. doi: 10.1016/0092-8674(94)90150-3.
Growth factors and cytokines are thought to influence the development of uncommitted progenitor cell populations, but the issue of how these factors act on individual cells remains controversial. Such factors may act simply as selective mitogens or survival factors for cells that undergo lineage restrictions stochastically. Alternatively, they may instruct or bias multipotent cells to choose one lineage at the expense of others. Here we show that glial growth factor (GGF), previously defined as a Schwann cell mitogen, strongly suppresses neuronal differentiation of rat neural crest stem cells while promoting or allowing glial differentiation. Quantitative clonal analysis suggests that the action of GGF is likely to be instructive rather than selective. Taken together with the expression pattern of GGF, these data suggest a lateral signaling model for the diversification of cell types within developing peripheral ganglia.
生长因子和细胞因子被认为会影响未分化祖细胞群体的发育，但这些因子如何作用于单个细胞的问题仍存在争议。这些因子可能仅仅作为随机经历谱系限制的细胞的选择性有丝分裂原或存活因子。或者，它们可能指导或偏向多能细胞以牺牲其他谱系为代价选择一个谱系。在这里，我们表明，先前被定义为施万细胞有丝分裂原的胶质生长因子（GGF）强烈抑制大鼠神经嵴干细胞的神经元分化，同时促进或允许胶质分化。定量克隆分析表明，GGF的作用可能是指导性的而非选择性的。结合GGF的表达模式，这些数据提示了一个关于发育中的外周神经节内细胞类型多样化的侧向信号模型。
