Echt C S, Kidwell K K, Knapp S J, Osborn T C, McCoy T J
Plant and Soil Science Department, Montana State University, Bozeman 59717-0312.
Genome. 1994 Feb;37(1):61-71. doi: 10.1139/g94-008.
A genome map of cultivated alfalfa was constructed using segregating restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNAs (RAPDs) in a diploid backcross population generated from noninbred parents. Among the 153 loci scored in 87 progeny, four segregation ratios were observed for codominant and dominant markers: 1:1, 1:2:1, 1:1:1:1, and 3:1. Deviations from expected Mendelian ratios (p < 0.05) were observed for 34% of the loci studied. A genome map was assembled from two separate linkage maps, each constructed from a subset of the segregation data. One linkage map was constructed from 46 RFLP and 40 RAPD markers segregating 1:1 from the F1 parent of the backcross and the other linkage map was constructed from 33 RFLP and 28 RAPD markers segregating 1:1 from the recurrent parent. Sixteen loci with alleles segregating 1:1 from both parents were used as locus bridges to align individual linkage groups between the two maps. The combined use of RFLPs and RAPDs was an effective method for developing an alfalfa genome map.
利用非近交亲本产生的二倍体回交群体中的分离限制性片段长度多态性（RFLP）和随机扩增多态性DNA（RAPD）构建了栽培苜蓿的基因组图谱。在87个后代中检测的153个基因座中，共显性和显性标记观察到四种分离比例：1:1、1:2:1、1:1:1:1和3:1。在所研究的基因座中，34%的基因座观察到偏离预期孟德尔比例（p<0.05）。基因组图谱由两个单独的连锁图谱组装而成，每个连锁图谱由分离数据的一个子集构建。一个连锁图谱由46个RFLP和40个RAPD标记构建，这些标记从回交的F1亲本中以1:1分离，另一个连锁图谱由33个RFLP和28个RAPD标记构建，这些标记从轮回亲本中以1:1分离。16个基因座的等位基因从双亲中以1:1分离，用作基因座桥梁来对齐两个图谱之间的各个连锁群。RFLP和RAPD的联合使用是构建苜蓿基因组图谱的有效方法。
