Giese H, Holm-Jensen A G, Mathiassen H, Kjaer B, Rasmussen S K, Bay H, Jensen J
Environmental Science and Technology Department, Risø National Laboratory, Roskilde, Denmark.
Hereditas. 1994;120(3):267-73. doi: 10.1111/j.1601-5223.1994.00267.x.
The RAPD technique was found to provide reliable genetic markers in barley. A linkage study of 23 RAPDs, 28 RFLPs, and 29 gene loci was conducted on 72 chromosome-doubled haploid progeny lines from a barley cross. The resulting linkage map covered 680 cM, about half of the barley genome. RAPD markers were distributed throughout the map, but a higher than expected frequency of tightly linked RAPDs was observed. Several cases of skewed segregation ratios were observed, but the RAPD markers segregated in ratios similar to their linked loci, confirming that they were reliably scored. In separate crosses, two amplified RAPD products, generated by different primers, were shown to reside in corresponding chromosomal positions. The RAPD markers seem a realistic alternative to RFLP markers in linkage analysis of barley.
研究发现，随机扩增多态性DNA（RAPD）技术可为大麦提供可靠的遗传标记。对大麦杂交产生的72个染色体加倍单倍体后代株系进行了23个RAPD标记、28个限制性片段长度多态性（RFLP）标记和29个基因位点的连锁研究。由此得到的连锁图谱覆盖了680厘摩（cM），约为大麦基因组的一半。RAPD标记分布于整个图谱，但观察到紧密连锁的RAPD出现频率高于预期。观察到几例偏分离比例的情况，但RAPD标记的分离比例与其连锁位点相似，证实了它们的评分可靠。在单独的杂交实验中，由不同引物产生的两个扩增RAPD产物显示位于相应的染色体位置。在大麦的连锁分析中，RAPD标记似乎是RFLP标记切实可行的替代物。
