Shorrosh B S, Dixon R A, Ohlrogge J B
Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824-1312.
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4323-7. doi: 10.1073/pnas.91.10.4323.
Acetyl-CoA carboxylase [ACCase; acetyl-CoA:carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] catalyzes the ATP-dependent carboxylation of acetyl CoA to produce malonyl CoA. In plants, malonyl CoA is needed for plastid localized fatty acid biosynthesis and for a variety of pathways in the cytoplasm including flavonoid biosynthesis. We have determined the full nucleotide sequence of an ACCase from alfalfa, which appears to represent a cytoplasmic isozyme. Partial cDNAs were isolated from a cDNA library of suspension culture cells that had been elicited for isoflavonoid phytoalexin synthesis. The full-length sequence was obtained by primer extension and amplification of the cDNA with synthetic primers. The sequence codes for a protein of 2257 amino acids with a calculated M(r) of 252,039. The biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase domains, respectively, show approximately 72%, 50%, and 65% sequence similarity to those of animal, diatom, and yeast ACCase sequences. ACCase enzyme activity and transcripts are induced severalfold upon addition of yeast or fungal elicitors to alfalfa cell cultures.
乙酰辅酶A羧化酶[ACCase；乙酰辅酶A：二氧化碳连接酶（生成ADP），EC 6.4.1.2]催化乙酰辅酶A的ATP依赖性羧化反应，生成丙二酰辅酶A。在植物中，丙二酰辅酶A是质体定位的脂肪酸生物合成以及细胞质中多种途径（包括类黄酮生物合成）所必需的。我们已经确定了来自苜蓿的一种ACCase的完整核苷酸序列，它似乎代表一种细胞质同工酶。从已诱导异黄酮植保素合成的悬浮培养细胞的cDNA文库中分离出部分cDNA。通过引物延伸和用合成引物扩增cDNA获得了全长序列。该序列编码一个2257个氨基酸的蛋白质，计算的M(r)为252,039。生物素羧化酶、生物素羧基载体蛋白和羧基转移酶结构域分别与动物、硅藻和酵母ACCase序列显示出约72%、50%和65%的序列相似性。在苜蓿细胞培养物中添加酵母或真菌激发子后，ACCase酶活性和转录本会被诱导增加数倍。
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