采用ATP细胞活力检测法比较紫杉醇和多西他赛（泰索帝）对妇科和乳腺癌细胞系的作用。

Comparison of paclitaxel and docetaxel (Taxotere) in gynecologic and breast cancer cell lines with the ATP-cell viability assay.

作者信息

Untch M, Untch A, Sevin B U, Angioli R, Perras J P, Koechli O, Averette H E

机构信息

Frauenklinik im Klinikum Grosshaderm, Ludwig Maximillians Universität, Grosshadern, Munich, Germany.

出版信息

Anticancer Drugs. 1994 Feb;5(1):24-30. doi: 10.1097/00001813-199402000-00004.

DOI:10.1097/00001813-199402000-00004

PMID:7910494

Abstract

The in vitro effects of paclitaxel (Tx) and docetaxel (Taxotere, Txt) are compared in this study using the adenosine triphosphate cell viability assay (ATP-CVA) in 14 cancer cell lines. Eleven cell lines were sensitive and three were partially sensitive to paclitaxel. Nine cell lines were sensitive, three were partially sensitive and two were resistant to docetaxel. Mean IC50s were 3.7-660 ng/ml paclitaxel and 5.4-540 ng/ml docetaxel. In five sensitive cancer cell lines docetaxel was more active than paclitaxel, and in six sensitive cell lines paclitaxel was more active than docetaxel on a concentration basis. Two cell lines were sensitive to paclitaxel and resistant to docetaxel. In one cell line the two compounds had similar activities. In the ATP-CVA, paclitaxel and docetaxel are very active and are partially non-cross-resistant.

摘要

在本研究中，使用三磷酸腺苷细胞活力测定法（ATP-CVA）在14种癌细胞系中比较了紫杉醇（Tx）和多西他赛（泰索帝，Txt）的体外效应。11种细胞系对紫杉醇敏感，3种对紫杉醇部分敏感。9种细胞系对多西他赛敏感，3种对多西他赛部分敏感，2种对多西他赛耐药。紫杉醇的平均半数抑制浓度（IC50）为3.7 - 660 ng/ml，多西他赛的平均半数抑制浓度为5.4 - 540 ng/ml。在5种敏感癌细胞系中，多西他赛比紫杉醇更具活性，在6种敏感细胞系中，基于浓度，紫杉醇比多西他赛更具活性。2种细胞系对紫杉醇敏感但对多西他赛耐药。在1种细胞系中，这两种化合物具有相似的活性。在ATP-CVA中，紫杉醇和多西他赛活性很强且部分存在非交叉耐药性。