Kwon S J, Park J W, Choi W K, Kim I H, Kim K
Department of Food and Nutrition, Chonnam National University, Kwangju, Korea.
Biochem Biophys Res Commun. 1994 May 30;201(1):8-15. doi: 10.1006/bbrc.1994.1662.
A protector protein from Saccharomyces cerevisiae specifically prevents the inactivation of enzymes caused by a thiol/Fe3+/O2 metal-catalyzed oxidation system but not by an ascorbate/Fe3+/O2 system. Ascorbate/Fe3+/O2-mediated damage of enzymes could be prevented by the protector protein only in the presence of reduced thiol. We demonstrate that two proteins from yeast, thioredoxin plus another protein having properties similar to that expected to thioredoxin reductase, when presented with NADPH and the yeast protector protein prevented inactivation of E. coli glutamine synthetase by the ascorbate/Fe3+/O2 system. This system also removes hydrogen peroxide effectively. We also demonstrate evidence suggesting that the NADPH-dependent thioredoxin system reactivates protector protein by reversible disulfide-dithiols exchange.
