Yoshpe-Purer Y, Henis Y
Appl Environ Microbiol. 1976 Oct;32(4):465-9. doi: 10.1128/aem.32.4.465-469.1976.
Composition of the culture medium, growth phase, and temperature play important roles in the sensitivity of Escherichia coli to H2O2. The medium and growth phase affected the sensitivity of the cells to H2O2 by modifying the amount of catalase synthesized by them, whereas the effect of temperature was due to the thermolability of the enzyme. Since catalase is unstable in the presence of its substrate, the correlation between the catalase level in the cells and their sensitivity to H2O2 could be observed only when the H2O2 concentration was not excessive in proportion to the amount of catalase.
培养基的成分、生长阶段和温度对大肠杆菌对过氧化氢的敏感性起着重要作用。培养基和生长阶段通过改变细胞合成过氧化氢酶的量来影响细胞对过氧化氢的敏感性，而温度的影响则是由于该酶的热不稳定性。由于过氧化氢酶在其底物存在时不稳定，只有当过氧化氢浓度与过氧化氢酶的量相比不过量时，才能观察到细胞中过氧化氢酶水平与其对过氧化氢敏感性之间的相关性。
