Rajan T V, Nelson F K, Killeen N, Shultz L D, Yates J A, Bailis J M, Littman D R, Greiner D L
Department of Pathology, University of Connecticut Health Center, Farmington 06030.
Exp Parasitol. 1994 Jun;78(4):352-60. doi: 10.1006/expr.1994.1038.
Immunocompetent mice are nonpermissive for the development and maturation of the human filarial parasite, Brugia malayi. We and others have shown that the absence of T-lymphocytes, alone or in combination with B-lymphocytes, renders mice permissive to infection. In a previous study, we showed that mice lacking CD8+ T-lymphocytes are also completely nonpermissive for B. malayi, indicating that CD8+ T-lymphocytes are not an obligate requirement for resistance. In the present study, we have examined the role of CD4+ T-lymphocytes in resistance to filarial infection using two experimental systems. In the first, we used an anti-CD4 monoclonal antibody to deplete CD4+ T-cells in vivo in immunocompetent BALB/c mice. In the second system, we used mutant mice in which the gene encoding the CD4 antigen had been disrupted by homologous recombination, resulting in a lack of CD4+ T-cells. Challenge of either the anti-CD4 antibody depleted BALB/c mice or CD4 knockout mice with B. malayi infective-stage larvae demonstrated that mice lacking CD4+ T-lymphocytes were resistant to infection. These data indicate that CD4+ T-cells are not an obligate requirement for murine resistance to B. malayi.
具有免疫活性的小鼠对人类丝虫寄生虫马来布鲁线虫的发育和成熟不具有易感性。我们和其他人已经表明，单独或与B淋巴细胞联合缺乏T淋巴细胞会使小鼠对感染具有易感性。在先前的一项研究中，我们表明缺乏CD8 + T淋巴细胞的小鼠对马来布鲁线虫也完全不具有易感性，这表明CD8 + T淋巴细胞不是抵抗所必需的。在本研究中，我们使用两个实验系统研究了CD4 + T淋巴细胞在抵抗丝虫感染中的作用。在第一个实验中，我们使用抗CD4单克隆抗体在具有免疫活性的BALB / c小鼠体内耗尽CD4 + T细胞。在第二个系统中，我们使用了突变小鼠，其中编码CD4抗原的基因已通过同源重组被破坏，导致缺乏CD4 + T细胞。用马来布鲁线虫感染期幼虫攻击抗CD4抗体耗尽的BALB / c小鼠或CD4基因敲除小鼠，结果表明缺乏CD4 + T淋巴细胞的小鼠对感染具有抵抗力。这些数据表明，CD4 + T细胞不是小鼠抵抗马来布鲁线虫所必需的。
