Miyakawa Y, Mabuchi T
Department of Microbiology, Yamanashi Medical College, Japan.
J Med Vet Mycol. 1994;32(1):71-5.
A species-specific DNA fragment EO3 isolated from Candida albicans was characterized. EO3 was shown to originate from a duplicated region of C. albicans mitochondrial (mt) DNA. Nucleotide sequence of a 0.9 kbp fragment (EO3S) which confers the species specificity of EO3 was determined. EO3S region in some strains contained two parts of deletions (or insertions) of 50-60 bp with short inverted repeats at both ends. The EO3S region was maintained stably in mtDNA of individual C. albicans strains and may be useful as target DNA for the diagnosis and epidemiological studies of candidiasis.
对从白色念珠菌中分离出的一个物种特异性DNA片段EO3进行了表征。结果表明EO3源自白色念珠菌线粒体（mt）DNA的一个重复区域。确定了赋予EO3物种特异性的0.9 kbp片段（EO3S）的核苷酸序列。一些菌株中的EO3S区域包含两个50 - 60 bp的缺失（或插入）部分，两端带有短反向重复序列。EO3S区域在单个白色念珠菌菌株的mtDNA中稳定存在，可能作为念珠菌病诊断和流行病学研究的靶DNA。
