白细胞介素-1增强新生T淋巴细胞中减少的白细胞介素-2 mRNA表达以及其对抗CD2抗体的增殖反应。

Interleukin-1 augments the diminished interleukin-2 mRNA expression and proliferative response of neonatal T lymphocytes to anti-CD2 antibodies.

作者信息

Hassan J, Reen D J

机构信息

Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin, Ireland.

出版信息

Scand J Immunol. 1994 Jun;39(6):597-601. doi: 10.1111/j.1365-3083.1994.tb03418.x.

DOI:10.1111/j.1365-3083.1994.tb03418.x

PMID:7912004

Abstract

Expression of IL-2 mRNA by unstimulated and stimulated purified T cells and mononuclear cells from adult and cord blood was investigated in an attempt to better understand the underlying defective neonatal host immune defense system. Using RNA dot-blot analysis, IL-2 mRNA expression in anti-CD2-stimulated neonatal T cells revealed significantly reduced levels when compared to adult T cells (P < 0.01). Purified neonatal T cells also showed a significantly reduced proliferative response to anti-CD2 antibodies (P < 0.01). Addition of IL-1 beta enhanced the hyporesponsiveness of neonatal T cells at both the level of proliferation and IL-2 mRNA expression. Unseparated mononuclear cells from adult and cord blood revealed similar IL-2 mRNA levels and proliferation when activated by anti-CD2 stimulation. The reduced IL-2 mRNA expression observed in neonatal T cells may explain, in part, the difference in host defense between the newborn and adult during states of increased demand such as infection.

摘要

为了更好地理解潜在的新生儿宿主免疫防御系统缺陷，我们研究了来自成人和脐血的未刺激及刺激后的纯化T细胞和单核细胞中IL-2 mRNA的表达情况。使用RNA斑点印迹分析，与成人T细胞相比，抗CD2刺激的新生儿T细胞中IL-2 mRNA表达水平显著降低（P < 0.01）。纯化的新生儿T细胞对抗CD2抗体的增殖反应也显著降低（P < 0.01）。添加IL-1β可增强新生儿T细胞在增殖水平和IL-2 mRNA表达方面的低反应性。来自成人和脐血的未分离单核细胞在抗CD2刺激下激活时，显示出相似的IL-2 mRNA水平和增殖情况。在新生儿T细胞中观察到的IL-2 mRNA表达降低可能部分解释了新生儿和成人在感染等需求增加状态下宿主防御的差异。