van Doorn J, Boonekamp P M, Oudega B
Bulb Research Centre, Department of Plant Quality, 2160 AB Lisse, Netherlands
Mol Plant Microbe Interact. 1994 May-Jun;7(3):334-44. doi: 10.1094/mpmi-7-0334.
Xanthomonas campestris pv. hyacinthi is a plant-pathogenic bacterium that causes yellow disease in Hyacinthus. X. c. pv.hyacinthi produces monopolarly attached fimbriae with a diameter of approximately 5 nm and a length of at least 6 micron. Fimbriae were purified by acid precipitation and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No hemagglutinating activity of purified fimbriae was found when the fimbriae were tested with several types of erythrocytes. The fimbrial protein subunit had a relative molecular mass of 17 kDa; an isoelectric point was found at pH 4.1. Analysis of the N-terminal amino acid sequence of the fimbrial subunit indicated that X. c. pv. hyacinthi expresses type 4 fimbriae. A polyclonal rabbit antiserum was raised against the purified fimbriae. This antiserum recognized fimbriae of X. c. pv. hyacinthi in immunogold electron microscopy and immunoblotting experiments. Immunofluorescence studies showed that X. c. pv. hyacinthi cells as well as purified native fimbriae were attached to stomata of hyacinth leaves, suggesting a role for these surface antigens in the first stages of yellow disease.
野油菜黄单胞菌风信子致病变种是一种植物致病细菌，可引起风信子黄化病。野油菜黄单胞菌风信子致病变种产生单极附着的菌毛，直径约5纳米，长度至少6微米。菌毛通过酸沉淀和制备性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行纯化。用几种类型的红细胞对纯化的菌毛进行测试时未发现其血凝活性。菌毛蛋白亚基的相对分子质量为17 kDa；在pH 4.1处发现了一个等电点。对菌毛亚基的N端氨基酸序列分析表明，野油菜黄单胞菌风信子致病变种表达4型菌毛。用纯化的菌毛制备了兔多克隆抗血清。在免疫金电子显微镜和免疫印迹实验中，该抗血清识别野油菜黄单胞菌风信子致病变种的菌毛。免疫荧光研究表明，野油菜黄单胞菌风信子致病变种细胞以及纯化的天然菌毛附着在风信子叶片的气孔上，表明这些表面抗原在黄化病初期发挥作用。
