Menichella G, Pierelli L, Vittori M, Serafini R, Foddai M L, Rossi P L, Leone G, Sica S, Scambia G, Benedetti Panici L
Research Center for the Manipulation of Blood Components, Sacro Cuore Catholic University, Roma, Italy.
Int J Artif Organs. 1993 Dec;16 Suppl 5:39-44.
Several authors have reported a faster immunological and hemopoietic post-transplant reconstitution using autologous peripheral blood stem cell (PBSC) than using autologous bone marrow stem cells. A large number of PBSC can be collected by leukapheresis during the hematological recovery after induction or salvage chemotherapy. In our experience we demonstrated that several separators, even if they have different results in mononuclear cell (MNC) yields, red blood cell and platelet contaminations, are able to collect PBSC for autotransplantation in patients with several malignant diseases and different status of disease. Eighty three patients were submitted to 590 leukapheresis procedures using 4 different blood cell separators: the results showed that all employed protocols are efficient in the collection of peripheral MNC even if after the widespread use of granulocytecolony stimulating factor (G-CSF) in the harvesting phase, the blood cell separator efficiency in terms of MNC is reduced. The use of GCSF in combination with other growth factors, during chemotherapy mobilization could simplify, in the future, this therapeutical program even if improvements in the efficiency of PBSC collection protocols are required.
几位作者报告称，与使用自体骨髓干细胞相比，使用自体外周血干细胞（PBSC）进行移植后免疫和造血重建的速度更快。在诱导或挽救性化疗后的血液学恢复期间，可以通过白细胞分离术采集大量的PBSC。根据我们的经验，我们证明，即使几种血细胞分离机在单核细胞（MNC）产量、红细胞和血小板污染方面有不同的结果，但它们都能够为患有几种恶性疾病和不同疾病状态的患者采集用于自体移植的PBSC。83名患者使用4种不同的血细胞分离机进行了590次白细胞分离术：结果表明，所有采用的方案在采集外周血MNC方面都是有效的，即使在采集阶段广泛使用粒细胞集落刺激因子（G-CSF）后，血细胞分离机在MNC方面的效率有所降低。在化疗动员期间，将GCSF与其他生长因子联合使用，未来可能会简化这个治疗方案，即使需要提高PBSC采集方案的效率。
