Menichella G, Lai M, Pierelli L, Vittori M, Serafini R, Ciarli M, Foddai M L, Salerno G, Sica S, Scambia G, Leone G, Bizzi B
Centro Ricerche per la Manipolazione dei Costituenti Ematici, Catholic University, Rome, Italy.
Vox Sang. 1997;73(4):230-6. doi: 10.1046/j.1423-0410.1997.7340230.x.
Reconstitution of hematopoiesis by means of peripheral blood stem cells is a valid alternative to autologous bone marrow transplantation. The aim of this investigation was to increase the efficiency of collection of circulating blood progenitor cells and to obtain a purer product for transplant.
We carried out leukapheresis procedures with the Fresenius AS 104 blood cell separator, using two different protocols, the previously used PBSC-LYM and a new mononuclear cell collection program.
Both programs were highly effective in collecting mononuclear cells (MNC) and CD34+ cells. Some differences were found, especially regarding MNC yield and efficiencies. There are remarkable differences in the efficiency of collection of CD34+ cells (62.38% with the new program as opposed to 31.69% with the older one). Linear regression analysis showed a negative correlation between blood volume processed and MNC efficiency only for the PBSC-LYM program. Differences were also observed in the degree of inverse correlation existing in both programs between patients' white blood cell precount and MNC collection efficiency. The inverse correlation was stronger for the PBSC-LYM program. Seven patients with solid tumors and hematologic malignancies received high dose chemotherapy and were subsequently transplanted with peripheral blood stem cells collected using the new protocol. All patients obtained a complete and stable engraftment with the reinfusion product collected with one or two leukapheresis procedures.
High efficiencies and yields were observed in the new protocol for MNC and CD34+ cells. These were able to effect rapid and complete bone marrow recovery after myeloablative chemotherapy.
通过外周血干细胞重建造血功能是自体骨髓移植的一种有效替代方法。本研究的目的是提高循环血祖细胞的采集效率，并获得更纯净的移植产品。
我们使用费森尤斯AS 104血细胞分离机进行白细胞单采程序，采用两种不同方案，即先前使用的PBSC-LYM方案和一种新的单核细胞采集程序。
两种方案在采集单核细胞（MNC）和CD34+细胞方面都非常有效。发现了一些差异，特别是在MNC产量和效率方面。CD34+细胞的采集效率存在显著差异（新方案为62.38%，而旧方案为31.69%）。线性回归分析显示，仅对于PBSC-LYM方案，处理的血量与MNC效率之间呈负相关。在两种方案中，患者白细胞预计数与MNC采集效率之间的负相关程度也存在差异。PBSC-LYM方案的负相关更强。7例实体瘤和血液系统恶性肿瘤患者接受了大剂量化疗，随后接受了使用新方案采集的外周血干细胞移植。所有患者通过一两次白细胞单采程序采集的回输产品均实现了完全且稳定的植入。
新方案在MNC和CD34+细胞的采集效率和产量方面表现出色。这些细胞能够在清髓性化疗后实现快速且完全的骨髓恢复。
