Dharakul T, Kurane I, Bhamarapravati N, Yoksan S, Vaughn D W, Hoke C H, Ennis F A
Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
J Infect Dis. 1994 Jul;170(1):27-33. doi: 10.1093/infdis/170.1.27.
A live attenuated dengue virus type 2 candidate vaccine (16681-PDK53) was evaluated in a phase I trial in 10 nonimmune adult volunteers. The dengue virus-specific memory T cell responses were analyzed as part of this study. Dengue virus-specific T cell proliferative responses were observed in all subjects after stimulating their peripheral blood mononuclear cells with live viruses or noninfectious viral antigens. The highest proliferative response was against dengue virus type 2, although cross-reactivity with other flaviviruses was detected to a lesser degree in some subjects. Dengue virus type 2-specific CD4+ and CD8+ cytotoxic T lymphocytes were generated in all vaccinees. This study investigated whether the candidate vaccine was efficacious in inducing dengue virus-specific CD4+ and CD8+ T cell memory after a single immunization in nonimmune recipients.
一种2型登革热病毒减毒活疫苗候选株（16681-PDK53）在10名未感染过登革热的成年志愿者中进行了I期试验评估。作为本研究的一部分，对登革热病毒特异性记忆T细胞反应进行了分析。在用活病毒或非感染性病毒抗原刺激外周血单个核细胞后，在所有受试者中均观察到登革热病毒特异性T细胞增殖反应。最高的增殖反应针对2型登革热病毒，尽管在一些受试者中检测到与其他黄病毒的交叉反应程度较低。所有接种疫苗者均产生了2型登革热病毒特异性CD4+和CD8+细胞毒性T淋巴细胞。本研究调查了该候选疫苗在未感染过登革热的受试者单次免疫后，是否能有效诱导登革热病毒特异性CD4+和CD8+T细胞记忆。
