Derouiche A, Ohm T G
Institute of Anatomy, J.W. Goethe University, Frankfurt am Main, Germany.
Neurosci Lett. 1994 Jan 3;165(1-2):179-82. doi: 10.1016/0304-3940(94)90739-0.
Vibratome sections of immersion-fixed (4% paraformaldehyde) hippocampi from four autoptic cases without neuropathological findings were immunostained with a polyclonal anti-glutamine synthetase antibody. Immunoreactivity is restricted to astrocytes and has a layer-specific distribution: The highest levels are observed in the outer molecular layer of the fascia dentata, in the pyramidal cell layer and in the upper stratum oriens. The stratum radiatum displays particularly low immunoreaction. A conspicuous drop in immunoreactivity from the heavily stained outer molecular layer to its inner portion is marked by a clear-cut dividing line. The layer-specific staining pattern found corresponds to the laminated distribution of glutamate binding sites described by others.
对4例无神经病理学发现的尸检病例的海马体（经4%多聚甲醛浸泡固定）进行振动切片，并用多克隆抗谷氨酰胺合成酶抗体进行免疫染色。免疫反应仅限于星形胶质细胞，且具有层特异性分布：在齿状回的外分子层、锥体细胞层和上原层中观察到最高水平。辐射层的免疫反应特别低。从染色浓重的外分子层到其内部，免疫反应明显下降，由一条清晰的分界线标记。所发现的层特异性染色模式与其他人描述的谷氨酸结合位点的分层分布相对应。
