食管鳞状细胞癌中的人乳头瘤病毒DNA序列

Human papillomavirus DNA sequences in esophagus squamous cell carcinoma.

作者信息

Togawa K, Jaskiewicz K, Takahashi H, Meltzer S J, Rustgi A K

机构信息

Massachusetts General Hospital, Harvard Medical School, Boston.

出版信息

Gastroenterology. 1994 Jul;107(1):128-36. doi: 10.1016/0016-5085(94)90070-1.

DOI:10.1016/0016-5085(94)90070-1

PMID:7912679

Abstract

BACKGROUND/AIMS: Esophagus squamous cell carcinoma has much geographic variation. A variety of genetic and environmental factors have been implicated in the pathogenesis of esophagus squamous cell carcinoma. This study was undertaken to determine whether the human papillomavirus is present in these tumors.

METHODS

A radioactive nested polymerase chain reaction was used to determine the presence of human papillomavirus in esophagus squamous cell carcinoma DNA and adjacent normal mucosa DNA from different regions of the world. Restriction fragment length polymorphism analysis was used to determine which particular human papillomavirus genotype was present.

RESULTS

Human papillomavirus was identified in 14% of esophageal squamous cell cancer DNA but in none of the adjacent normal mucosa DNA available for some of the samples. Positive samples were found to contain sequences specific for high-risk human papillomaviruses, either types 16 or 18. In addition, a novel human papillomavirus genotype was detected in another 10% of the samples.

CONCLUSIONS

Esophagus squamous cell carcinoma is associated with the expression of human papillomavirus genotypes 16 and 18, which are linked to transformation of squamous epithelial cells. In addition, a novel human papillomavirus genotype that was identified may be associated with pathogenesis in esophagus squamous cell cancer.

摘要

背景/目的：食管鳞状细胞癌存在显著的地域差异。多种遗传和环境因素被认为与食管鳞状细胞癌的发病机制有关。本研究旨在确定这些肿瘤中是否存在人乳头瘤病毒。