Togawa K, Jaskiewicz K, Takahashi H, Meltzer S J, Rustgi A K
Massachusetts General Hospital, Harvard Medical School, Boston.
Gastroenterology. 1994 Jul;107(1):128-36. doi: 10.1016/0016-5085(94)90070-1.
BACKGROUND/AIMS: Esophagus squamous cell carcinoma has much geographic variation. A variety of genetic and environmental factors have been implicated in the pathogenesis of esophagus squamous cell carcinoma. This study was undertaken to determine whether the human papillomavirus is present in these tumors.
A radioactive nested polymerase chain reaction was used to determine the presence of human papillomavirus in esophagus squamous cell carcinoma DNA and adjacent normal mucosa DNA from different regions of the world. Restriction fragment length polymorphism analysis was used to determine which particular human papillomavirus genotype was present.
Human papillomavirus was identified in 14% of esophageal squamous cell cancer DNA but in none of the adjacent normal mucosa DNA available for some of the samples. Positive samples were found to contain sequences specific for high-risk human papillomaviruses, either types 16 or 18. In addition, a novel human papillomavirus genotype was detected in another 10% of the samples.
Esophagus squamous cell carcinoma is associated with the expression of human papillomavirus genotypes 16 and 18, which are linked to transformation of squamous epithelial cells. In addition, a novel human papillomavirus genotype that was identified may be associated with pathogenesis in esophagus squamous cell cancer.
背景/目的：食管鳞状细胞癌存在显著的地域差异。多种遗传和环境因素被认为与食管鳞状细胞癌的发病机制有关。本研究旨在确定这些肿瘤中是否存在人乳头瘤病毒。
采用放射性巢式聚合酶链反应来检测来自世界不同地区的食管鳞状细胞癌DNA及相邻正常黏膜DNA中人乳头瘤病毒的存在情况。利用限制性片段长度多态性分析来确定存在哪种特定的人乳头瘤病毒基因型。
在14%的食管鳞状细胞癌DNA中检测到人乳头瘤病毒，但部分样本的相邻正常黏膜DNA中均未检测到。阳性样本被发现含有高危人乳头瘤病毒16型或18型的特异性序列。此外，在另外10%的样本中检测到一种新的人乳头瘤病毒基因型。
食管鳞状细胞癌与人乳头瘤病毒16型和18型的表达相关，这两种病毒与鳞状上皮细胞的转化有关。此外，鉴定出的一种新的人乳头瘤病毒基因型可能与食管鳞状细胞癌的发病机制有关。
