Schrenzel M D, Watson J L, Ferrick D A
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Immunogenetics. 1994;40(2):135-44. doi: 10.1007/BF00188177.
Genes encoding the horse (Equus caballus) T-cell receptor beta chain (TCRB) were cloned and characterized. Of 33 cDNA clones isolated from the mesenteric lymph node, 30 had functionally rearranged gene segments, and three contained germline sequences. Sixteen unique variable segments (TCRBV), 14 joining genes (TCRBJ), and two constant region genes (TCRBC) were identified. Horse TCRBV were grouped into nine families based on similarity to human sequences. TCRBV2 and TCRBV12 were the most commonly represented horse families. Analysis of predicted protein structure revealed the presence of conserved regions similar to those seen in TCRB of other species. A decanucleotide promoter sequence homologous to those found in humans and mice was located in the 5' untranslated region of one horse gene. Germline sequences included the 5' region of the TCRBD2 gene with flanking heptamer/nonamer recombination signals and portions of the TCRBJ2-C2 intron. Southern blot hybridizations demonstrated restriction fragment length polymorphisms at the TCRBC locus among different horse breeds.
对编码马（马属动物）T细胞受体β链（TCRB）的基因进行了克隆和特征分析。从肠系膜淋巴结分离出的33个cDNA克隆中，30个具有功能重排的基因片段，3个包含种系序列。鉴定出16个独特的可变区基因片段（TCRBV）、14个连接基因（TCRBJ）和2个恒定区基因（TCRBC）。基于与人类序列的相似性，马TCRBV被分为9个家族。TCRBV2和TCRBV12是马中最常见的家族。对预测的蛋白质结构分析表明，存在与其他物种TCRB中所见相似的保守区域。在一个马基因的5'非翻译区发现了与人类和小鼠中发现的十核苷酸启动子序列同源的序列。种系序列包括TCRBD2基因的5'区域，其侧翼为七聚体/九聚体重组信号以及TCRBJ2-C2内含子的部分序列。Southern印迹杂交显示不同马品种在TCRBC基因座处存在限制性片段长度多态性。
