Ikuta K, Ogura T, Shimizu A, Honjo T
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7701-5. doi: 10.1073/pnas.82.22.7701.
We have cloned three pairs of rearranged and germ-line variable region (V beta) genes of the beta chain of the human T-cell receptor from the cell lines ATL2, ATL12, and MT-1 of patients with adult T-cell leukemia (ATL). The pairs were derived from the same (for ATL2 and ATL12) and different (for MT-1) individuals. Comparison of the nucleotide sequences showed no somatic mutation in V beta X ATL2 and beta X ATL12-2. Although one nucleotide change was found in V beta X MT1-1, the possibility of polymorphism was not excluded. These results indicate that the frequency of somatic mutation in the V beta gene of the T-cell receptor is 1/10th or less than that in the immunoglobulin gene. Both alleles of the rearranged T-cell receptor gene were analyzed for ATL12 and MT-1. In both, only one of the two rearranged J beta alleles was an active variable-diversity-joining (V-D-J) complex. The results suggest that allelic exclusion occurs in the T-cell receptor gene.
我们从成人T细胞白血病（ATL）患者的细胞系ATL2、ATL12和MT-1中克隆了三对人T细胞受体β链重排的和种系可变区（Vβ）基因。这些基因对来自相同个体（ATL2和ATL12）以及不同个体（MT-1）。核苷酸序列比较显示，VβX ATL2和βX ATL12 - 2中没有体细胞突变。虽然在VβX MT1 - 1中发现了一个核苷酸变化，但不排除多态性的可能性。这些结果表明，T细胞受体Vβ基因中体细胞突变的频率是免疫球蛋白基因的十分之一或更低。对ATL12和MT-1的重排T细胞受体基因的两个等位基因都进行了分析。在两者中，两个重排的Jβ等位基因中只有一个是活跃的可变区-多样性区-连接区（V-D-J）复合体。结果表明，T细胞受体基因中存在等位基因排斥现象。
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