HIV-1糖蛋白41增强H9和U937细胞上主要组织相容性复合体I类分子和细胞间黏附分子-1的表达。

HIV-1 gp41 enhances major histocompatibility complex class I and ICAM-1 expression on H9 and U937 cells.

作者信息

Chen Y H, Böck G, Vornhagen R, Steindl F, Katinger H, Dierich M P

机构信息

Ludwig Boltzmann Institute for AIDS Research, Innsbruck, Austria.

出版信息

Int Arch Allergy Immunol. 1994 Jul;104(3):227-31. doi: 10.1159/000236670.

DOI:10.1159/000236670

PMID:7913356

Abstract

Based on our findings that HIV-1 (human immunodeficiency virus type 1) soluble gp41 (sgp41; amino acids 539-684) bound to human T, B, and monocyte cells and enhanced major histocompatibility complex (MHC) class I and II antigen expression on Raji cells, we examined the effect of HIV-1 sgp41 on the surface expression of MHC I and II, ICAM-1, and CD4 molecules on human H9 and U937 cells. Flow cytometry (FACS) analysis demonstrated that sgp41 selectively enhanced MHC class I expression by about 75% on H9 cells and by about 85% on U937 cells, while the ICAM-1 expression was increased by about 70% only on H9 cells and remained unaltered on U937 cells; other molecules, such as MHC class II and CD4, remained unaltered. By comparison, alpha-, beta-, and omega-interferons, but not gamma-interferon, showed similar effects as sgp41 on the expression of MHC class I and ICAM-1 on H9 and U937 cells. The results suggest that HIV-1 gp41 may have a biological function that is involved in the regulation of human MHC class I and ICAM-1 expression.

摘要

基于我们的研究发现，即HIV-1（人类免疫缺陷病毒1型）可溶性糖蛋白41（sgp41；氨基酸539 - 684）可与人T细胞、B细胞和单核细胞结合，并增强Raji细胞上主要组织相容性复合体（MHC）I类和II类抗原的表达，我们研究了HIV-1 sgp41对人H9和U937细胞表面MHC I类和II类、细胞间黏附分子-1（ICAM-1）以及CD4分子表达的影响。流式细胞术（FACS）分析表明，sgp41可使H9细胞上的MHC I类表达选择性增强约75%，U937细胞上增强约85%；ICAM-1表达仅在H9细胞上增加约70%，在U937细胞上保持不变；其他分子，如MHC II类和CD4，则保持不变。相比之下，α-、β-和ω-干扰素（而非γ-干扰素）对H9和U937细胞上MHC I类和ICAM-1的表达显示出与sgp41类似的作用。结果表明，HIV-1 gp41可能具有参与调节人类MHC I类和ICAM-1表达的生物学功能。