Analysis of the kinetics of photoinduced crosslinkage of the protein and RNA components of the Escherichia coli 50 S ribosomal subunit.

The kinetics of photoinduced crosslinkage of the protein and ribosomal RNA components of the Escherichia coli 50 S ribosomal subunit were determined by two-dimensional gel electrophoresis. One group of ribosomal proteins was found to be reactive in the crosslinkage reaction at doses of 254 nm radiation of 2.9 X 10(3) ergs-mm-2. A second group of 50-S ribosomal proteins was found to be unreactive in the crosslinkage reaction at doses of 254 nm radiation less than 8.6 X 10(6) ergs-mm-2, but were reactive at higher doses.