Imaizumi M, Suzuki T, Machida H, Onodera K
Biology Laboratory, Yamasa Corporation, Choshi, Japan.
Nihon Shinkei Seishin Yakurigaku Zasshi. 1994 Apr;14(2):83-91.
We developed a fully automated light/dark apparatus which detects locomotion, rearing and time spent in light and dark zones, and shuttle crossing of mice. This apparatus is controlled by a personal computer and detects these parameters by using infrared beamsensors. We used this apparatus to investigate the effects of the anxiolytics, diazepam (DZP) and pentobarbital (PB); the putative anxiolytic, 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT); and the anxiogenics, picrotoxin, methyl-beta-carboline-3-carboxylate (beta-CCM), and ethyl-beta-carboline-3-carboxylate (beta-CCE), on each parameter measured by a light/dark test in mice. DZP (5 mg/kg) and 8-OH-DPAT (0.1 and 0.2 mg/kg) significantly increased the time spent in the light zone. PB (10 mg/kg) also significantly increased the number of shuttle crossings between the dark and light zones. Conversely, picrotoxin (1 and 2 mg/kg) significantly decreased locomotion, rearing and time spent in the light zone. beta-CCM (10 mg/kg) and beta-CCE (5 mg/kg) also significantly decreased rearing and time spent in the light zone. Although the same dose of beta-CCE significantly decreased shuttle crossing and rearing in the dark zone, beta-CCE did not change locomotion in either zone. Our results indicate that this apparatus is useful for the assessment of the anxiolytic and anxiogenic activities of drugs in mice.
我们开发了一种全自动明暗箱装置，该装置可检测小鼠的运动、直立行为以及在明区和暗区所花费的时间，还有小鼠的穿梭次数。此装置由个人计算机控制，并通过使用红外光束传感器来检测这些参数。我们使用该装置研究了抗焦虑药地西泮（DZP）和戊巴比妥（PB）；假定的抗焦虑药8-羟基-2-（二正丙基氨基）四氢萘（8-OH-DPAT）；以及致焦虑剂印防己毒素、甲基-β-咔啉-3-羧酸酯（β-CCM）和乙基-β-咔啉-3-羧酸酯（β-CCE）对小鼠明暗试验所测量的各项参数的影响。DZP（5毫克/千克）和8-OH-DPAT（0.1和0.2毫克/千克）显著增加了小鼠在明区所花费的时间。PB（10毫克/千克）也显著增加了小鼠在暗区和明区之间的穿梭次数。相反，印防己毒素（1和2毫克/千克）显著减少了小鼠的运动、直立行为以及在明区所花费的时间。β-CCM（10毫克/千克）和β-CCE（5毫克/千克）也显著减少了小鼠的直立行为以及在明区所花费的时间。尽管相同剂量的β-CCE显著减少了小鼠在暗区的穿梭次数和直立行为，但β-CCE在两个区域均未改变小鼠的运动。我们的结果表明，该装置可用于评估药物在小鼠中的抗焦虑和致焦虑活性。
