巨细胞动脉炎发病时使用选定的TCR V和J基因片段进行CD4+和CD8+ T细胞扩增。
CD4+ and CD8+ T cell expansions using selected TCR V and J gene segments at the onset of giant cell arteritis.
作者信息
Grunewald J, Andersson R, Rydberg L, Gigliotti D, Schaufelberger C, Hansson G K, Wigzell H
机构信息
Microbiology and Tumor Biology Center (MTC), Karolinska Institute, Stockholm, Sweden.
出版信息
Arthritis Rheum. 1994 Aug;37(8):1221-7. doi: 10.1002/art.1780370817.
OBJECTIVE
To investigate T cell receptor (TCR) V alpha/V beta (and in selected cases, J beta) usage in CD4+ and CD8+ peripheral blood lymphocytes of patients with giant cell arteritis (GCA), before and after treatment, as well as to analyze the HLA types of these patients.
METHODS
Flow cytometry, with 10 anti-TCR V-specific monoclonal antibodies (MAb), was used. To analyze J beta usage by cell populations expressing certain V beta, we used the polymerase chain reaction (PCR) technique, with V beta- and C beta-specific primers, Southern blotting of PCR products, and subsequent hybridization with radiolabeled J beta-specific probes. HLA typing was performed using the microlymphocytotoxicity technique.
RESULTS
Seven of the 9 GCA patients had increased anti-TCR V MAb reactivities (interpreted as T cell expansions), which in many cases, correlated with clinical signs of disease. A strict preference for particular J beta segments was found in 3 of 3 expanded CD4+ T cell populations.
CONCLUSION
T lymphocytes expressing specific antigen receptors are implicated in the pathogenesis of GCA.
目的
研究巨细胞动脉炎（GCA）患者治疗前后CD4⁺和CD8⁺外周血淋巴细胞中T细胞受体（TCR）Vα/Vβ（以及部分病例中的Jβ）的使用情况，并分析这些患者的HLA类型。
方法
采用流式细胞术，使用10种抗TCR V特异性单克隆抗体（MAb）。为分析表达特定Vβ的细胞群体中Jβ的使用情况，我们使用聚合酶链反应（PCR）技术，采用Vβ和Cβ特异性引物，对PCR产物进行Southern印迹，并随后与放射性标记的Jβ特异性探针杂交。使用微量淋巴细胞毒性技术进行HLA分型。
结果
9例GCA患者中有7例抗TCR V MAb反应性增加（解释为T细胞扩增），在许多情况下，这与疾病的临床症状相关。在3个扩增的CD4⁺ T细胞群体中的3个中发现了对特定Jβ片段的严格偏好。
结论
表达特定抗原受体的T淋巴细胞与GCA的发病机制有关。
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