Hernádi L
Balaton Limnological Research Institute, Hungarian Academy of Sciences, Tihany.
Cell Tissue Res. 1994 Jul;277(1):189-98. doi: 10.1007/BF00303096.
Gamma-aminobutyric acid (GABA)-like immunoreactive neurons were studied in the central and peripheral nervous system of Helix pomatia by applying immunocytochemistry on whole-mount preparations and serial paraffin sections. GABA-immunoreactive cell bodies were found in the buccal, cerebral and pedal ganglia, but only GABA-immunoreactive fibers were found in the viscero-parietal-pleural ganglion complex. The majority of GABA-immunoreactive cell bodies were located in the pedal ganglia but a few could be found in the buccal ganglia. Varicose GABA-ir fibers could be seen in the neuropil areas and in distinct areas of the cell body layer of the ganglia. The majority of GABA-ir axonal processes run into the connectives and commissures of the ganglia, indicating an important central integrative role of GABA-immunoreactive neurons. GABA may also have a peripheral role, since GABA-immunoreactive fibers could be demonstrated in peripheral nerves and the lips. Glutamate injection did not change the number or distribution of GABA-immunoreactive neurons, but induced GABA immunoreactivity in elements of the connective tissue ensheathing the muscle cells and fibers of the buccal musculature. This shows that GABA may be present in different non-neural tissues as a product of general metabolic pathways.
通过对整装标本和连续石蜡切片进行免疫细胞化学研究，在罗马蜗牛的中枢和外周神经系统中对γ-氨基丁酸（GABA）样免疫反应性神经元进行了研究。在颊神经节、脑神经节和足神经节中发现了GABA免疫反应性细胞体，但在内脏-顶叶-胸膜神经节复合体中仅发现了GABA免疫反应性纤维。大多数GABA免疫反应性细胞体位于足神经节，但在颊神经节中也能发现少数。在神经纤维网区域以及神经节细胞体层的不同区域可见有曲张的GABA免疫反应性纤维。大多数GABA免疫反应性轴突进入神经节的连接部和连合部，表明GABA免疫反应性神经元具有重要的中枢整合作用。GABA可能也具有外周作用，因为在外周神经和唇部可显示出GABA免疫反应性纤维。谷氨酸注射并未改变GABA免疫反应性神经元的数量或分布，但在包绕颊部肌肉组织的肌细胞和纤维的结缔组织成分中诱导出了GABA免疫反应性。这表明GABA可能作为一般代谢途径的产物存在于不同的非神经组织中。
