Scofield R H, Frank M B, Neas B R, Horowitz R M, Hardgrave K L, Fujisaku A, McArthur R, Harley J B
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City.
Clin Immunol Immunopathol. 1994 Sep;72(3):335-41. doi: 10.1006/clin.1994.1150.
The immunogenetics of the autoantibody response to Ro (or SS-A) have been explored in patients with systemic lupus erythematous. Data show that alleles of the T cell beta receptor and HLA-DQ loci are cooperatively associated with the presence of anti-Ro autoantibodies in systemic lupus erythematosus. Identification of HLA-DQ by oligonucleotide probe binding to polymerase chain reaction products demonstrates that the combination of DQB10201 and one of DQA10101, DQA10102, or DQA10103 is associated with anti-Ro. Patients possessing a particular pair of T cell receptor beta restriction enzyme polymorphisms along with these specific HLA-DQ alleles produce quantitatively more anti-Ro as measured by a sensitive solid-phase immunoassay than patients without these T cell receptor and DQ alleles. Other work has shown that the autoimmune response is directed against the human Ro antigen. These results are consistent with a central role in the disregulation of autoimmunity involving a trimolecular complex composed of the autoantigen bound by a HLA-DQ molecule which, together, are bound in turn by T cells which express a particular subset of T cell receptors.
在系统性红斑狼疮患者中，已对针对Ro（或SS - A）自身抗体反应的免疫遗传学进行了研究。数据表明，T细胞β受体和HLA - DQ基因座的等位基因与系统性红斑狼疮中抗Ro自身抗体的存在协同相关。通过寡核苷酸探针与聚合酶链反应产物结合来鉴定HLA - DQ，结果显示DQB10201与DQA10101、DQA10102或DQA10103中的一个组合与抗Ro相关。与没有这些T细胞受体和DQ等位基因的患者相比，拥有特定一对T细胞受体β限制性内切酶多态性以及这些特定HLA - DQ等位基因的患者，通过灵敏的固相免疫测定法检测到，其产生的抗Ro在数量上更多。其他研究表明，自身免疫反应针对的是人类Ro抗原。这些结果与自身免疫失调中的核心作用一致，这种失调涉及一种由HLA - DQ分子结合自身抗原形成的三分子复合物，而该复合物又依次被表达特定T细胞受体亚群的T细胞所结合。
