Zhang X F, Fan M Y, Chen J, Bi D Z
Department of Rickettsiology, Chinese Academy of Preventive Medicine, Beijing.
Acta Virol. 1994 Feb;38(1):35-7.
Restriction endonuclease fragment length polymorphism (RFLP) analysis and polymerase chain reaction (PCR) were used to characterize the genotypic diversity of 7 strains of Rickettsia conorii from South Africa, Ethiopia, Morocco, India and Russia. The strains of R. conorii were divided into four genotypes by Rsa I or Pst I endonuclease digestion of PCR-amplified rickettsial DNA using primers derived from the R. rickettsii 190 K antigen gene. M-1 and Barbash strains were genotypically identical, but different from Indian, Ethiopian and S7 strains, which formed another group. Simko and Moroccan strains were genotypically different from each other and also from other strains of R. conorii. We conclude that there exist a genotypic diversity among intraspecies of R. conorii.
采用限制性内切酶片段长度多态性（RFLP）分析和聚合酶链反应（PCR）对来自南非、埃塞俄比亚、摩洛哥、印度和俄罗斯的7株康氏立克次体的基因型多样性进行了表征。利用源自立氏立克次体190K抗原基因的引物，通过对PCR扩增的立克次体DNA进行Rsa I或Pst I内切酶消化，将康氏立克次体菌株分为四种基因型。M-1和巴尔巴什菌株基因型相同，但与印度、埃塞俄比亚和S7菌株不同，后三者形成另一组。辛科菌株和摩洛哥菌株基因型彼此不同，也与其他康氏立克次体菌株不同。我们得出结论，康氏立克次体种内存在基因型多样性。
