Alteration in oxidative metabolism of alanine in cerebellar granule cell cultures as a consequence of the development of the ability to utilize alanine as an amino group donor for synthesis of transmitter glutamate.

Formation of 14CO2 from labeled alanine was measured in cultured cerebellar granule cells grown in the combined presence of alanine, alpha-ketoglutarate and glutamine or in the presence of glutamine alone. This was done in order to study whether the utilization of alpha-ketoglutarate plus alanine as precursors of transmitter glutamate, induced by culturing in the presence of these compounds, is reflected by an increase of CO2 production from alanine during stimulation with an elevated extracellular potassium concentration. Potassium stimulated CO2 production from alanine was present only in the cells grown in the combined presence of alanine, alpha-ketoglutarate and glutamine. This stimulation was abolished by glutamine, but not by ouabain, indicating that the depolarizing-induced stimulation of alanine metabolism is a consequence of increased release of transmitter glutamate formed from alanine, not a simple result of an increased metabolic rate.