Boon M E, Luzzatto R, Beck S, Bosch M M, Hermans J, Rietveld W J
Leiden Cytology and Pathology Laboratory, The Netherlands.
Pathol Res Pract. 1994 Apr;190(4):372-7. doi: 10.1016/S0344-0338(11)80409-7.
Since immunohistochemical detection of proliferating cell nuclear antigen (PCNA) is closely related to the cell cycle, this method can be used to visualize proliferative activity in paraffin sections of benign and premalignant cervical lesions. This was done in two types of benign lesions, immature and mature metaplasia, and the three types of CIN (I, II, and III). PCNA staining was assessed as heavy, faint, or negative nuclei in the deep, middle, and superficial layers of the epithelium. In the analysis of variance, significant differences between the three layers and between the five groups were found. The PCNA values can be helpful to distinguish immature metaplasia from CIN I. The values for PCNA-positive (heavy+faint) for an individual lesion at the three layers constitute its proliferation profile. The PCNA profiles of the CIN lesions differ fundamentally from the metaplasia profiles. It is conceivable that for an individual CIN lesion, PCNA staining might be prognostically more valuable than its CIN grade.
由于增殖细胞核抗原（PCNA）的免疫组织化学检测与细胞周期密切相关，该方法可用于观察良性和癌前宫颈病变石蜡切片中的增殖活性。对两种良性病变（未成熟化生和成熟化生）以及三种类型的宫颈上皮内瘤变（CIN I、II和III）进行了此项研究。PCNA染色评估为上皮深层、中层和表层细胞核染色深、淡或阴性。在方差分析中，发现三层之间以及五组之间存在显著差异。PCNA值有助于区分未成熟化生和CIN I。单个病变在三层的PCNA阳性（深染+淡染）值构成其增殖谱。CIN病变的PCNA谱与化生谱有根本差异。可以想象，对于单个CIN病变，PCNA染色在预后方面可能比其CIN分级更有价值。
