Mittal K R, Demopoulos R I, Goswami S
Department of Pathology, New York University Medical Center, New York.
Am J Surg Pathol. 1993 Feb;17(2):117-22. doi: 10.1097/00000478-199302000-00003.
Expression of Proliferating cell nuclear antigen (PCNA) was evaluated in formalin-fixed, paraffin-embedded normal and abnormal cervical squamous epithelia using immunoperoxidase stains and PC10 monoclonal antibody to PCNA. PCNA was exclusively expressed in the parabasal and basal layers of normal ectocervix and a similar pattern was seen in nine of the 11 cases with squamous metaplasia. Examples of cervical dysplasia showed expression in higher layers of cervical epithelium, corresponding to the degree of dysplasia. Increased staining was seen in condylomas and markedly reduced staining with atrophy. The percentage of basal cells that stained increased progressively from atrophic to normal, to condylomatous, to dysplastic epithelia. Proliferative activity can be satisfactorily assessed in formalin-fixed cervical epithelia using PC10 PCNA antibody. This assessment can be of potential diagnostic use in difficult cases.
采用免疫过氧化物酶染色法及PC10抗增殖细胞核抗原（PCNA）单克隆抗体，对福尔马林固定、石蜡包埋的正常和异常宫颈鳞状上皮中PCNA的表达进行评估。PCNA仅在正常宫颈外口的副基底层和基底层表达，11例鳞状化生病例中有9例呈现类似模式。宫颈发育异常的病例显示，宫颈上皮较高层出现表达，且与发育异常程度相对应。尖锐湿疣中可见染色增强，萎缩时染色明显减弱。从萎缩性上皮到正常上皮、再到湿疣性上皮和发育异常上皮，染色的基底细胞百分比逐渐增加。使用PC10 PCNA抗体可在福尔马林固定的宫颈上皮中令人满意地评估增殖活性。该评估在疑难病例中可能具有潜在的诊断用途。
