Gulati S C, Acaba L
Cornell Medical Center, Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
J Hematother. 1993 Winter;2(4):467-71. doi: 10.1089/scd.1.1993.2.467.
The success of autologous stem cell transplantation using bone marrow or peripheral blood stem cells depends not only on in vivo irradication of the disease by cytotoxic therapy but also on complete hematopoietic engraftment with no risk of relapse from the infused cells. Various methods are available for removing (purging) such contaminants. Purging techniques were developed by use of various in vitro models utilizing cell lines and fresh cancer cells. Once effective conditions were developed, they were advanced onto clinical trials. Various approaches for purging have been used. Subgroups of leukemia and lymphoma have now shown clinical benefit by the use of purged stem cells. Emphasis is now being placed on utilizing the information already obtained by the pre-clinical investigators on designing better clinical trials.
使用骨髓或外周血干细胞进行自体干细胞移植的成功不仅取决于通过细胞毒性疗法在体内根除疾病，还取决于完全的造血植入且不存在输注细胞复发的风险。有多种方法可用于去除（清除）此类污染物。清除技术是通过利用各种使用细胞系和新鲜癌细胞的体外模型开发的。一旦开发出有效的条件，就会推进到临床试验阶段。已经使用了各种清除方法。现在白血病和淋巴瘤的亚组通过使用清除后的干细胞已显示出临床益处。目前正着重利用临床前研究人员已经获得的信息来设计更好的临床试验。
