Goraczniak R M, Duda T, Sitaramayya A, Sharma R K
Unit of Regulatory and Molecular Biology, Pennsylvania College of Optometry, Philadelphia 19141.
Biochem J. 1994 Sep 1;302 ( Pt 2)(Pt 2):455-61. doi: 10.1042/bj3020455.
In the vertebrate photoreceptor cell, rod outer segment (ROS) is the site of visual signal-transduction process, and a pivotal molecule that regulates this process is cyclic GMP. Cyclic GMP controls the cationic conductance into the ROS, and light causes a decrease in the conductance by activating hydrolysis of the cyclic nucleotide. The identity of the granylate cyclase (ROS-GC) that synthesizes this pool of cyclic GMP is unknown. We now report the cloning, expression and functional characterization of a DNA from bovine retina that encodes ROS-GC.
在脊椎动物光感受器细胞中，视杆细胞外段（ROS）是视觉信号转导过程发生的部位，而调节这一过程的关键分子是环鸟苷酸（cGMP）。cGMP控制阳离子进入ROS的电导，而光通过激活环核苷酸的水解作用导致电导降低。合成这一cGMP池的鸟苷酸环化酶（ROS-GC）的身份尚不清楚。我们现在报告从牛视网膜中克隆、表达并对编码ROS-GC的DNA进行功能特性分析。
