Gutierrez C, Papamichail M, Embling P, Holborow E J
Eur J Immunol. 1976 Jul;6(7):477-80. doi: 10.1002/eji.1830060706.
The effect of nonspecific mitogens on the trapping of 125I-labeled aggregated human IgG (125I-AHGG) in germinal centers (GC) of mouse spleens has been investigated by both radioactivity uptake and immunofluorescence. Phytohemagglutinin and concanavalin A (Con A) significantly decreased trapping. Lipopolysaccharide produced less inhibition, and pokeweed mitogen had no significant effect. The maximum inhibition occurred with 250--500 mug Con A. This had no effect on 125I-AHGG uptake in liver kidney and blood. No differences were found between i.p. and i.v. routes of Con A injection. The effect of mitogens on the 125I-AHGG trapping in GC is due more likely to modification of the migratory properties of lymphocytes brought about by surface binding, than to their mitogenic properties, since Con A decreased 125I-AHGG localization in thymectomized, x-irradiated and bone marrow-reconstituted animals.
通过放射性摄取和免疫荧光法，研究了非特异性丝裂原对小鼠脾脏生发中心（GC）中125I标记的聚合人IgG（125I-AHGG）捕获的影响。植物血凝素和刀豆球蛋白A（Con A）显著降低了捕获率。脂多糖产生的抑制作用较小，而商陆丝裂原无显著影响。250-500μg Con A产生最大抑制作用。这对125I-AHGG在肝脏、肾脏和血液中的摄取无影响。Con A腹腔注射和静脉注射途径之间未发现差异。丝裂原对GC中125I-AHGG捕获的影响更可能是由于表面结合引起的淋巴细胞迁移特性的改变，而不是它们的促有丝分裂特性，因为Con A降低了在胸腺切除、X射线照射和骨髓重建动物中125I-AHGG的定位。
