Austruy E, Cohen-Salmon M, Antignac C, Béroud C, Henry I, Nguyen V C, Brugières L, Junien C, Jeanpierre C
INSERM U73, Château de Longchamp, Paris, France.
Cancer Res. 1993 Jun 15;53(12):2888-94.
We applied a subtractive hybridization approach to isolate genes differentially expressed between mature kidney and Wilms' tumor. We constructed a complementary DNA library from a total mature kidney complementary DNA subtracted by an excess of mRNA from a Wilms' tumor, WAGR4, with a germline deletion of 11p13 and a somatic loss of alleles at 11p15. Six clones presenting a differential pattern of expression, positive with mRNA from the mature kidney and negative with mRNA from the Wilms' tumor WAGR4, were characterized. Among these clones were two as yet unknown expressed sequences (D11S877E and D15S109E) and four sequences from known genes: renal dipeptidase (DPEP1), alpha B-crystallin (CRYA2), uromodulin (UMOD), and plasma glutathione peroxidase (GPX2). The different patterns of expression of these genes in 11 Wilms' tumors, whether or not they are hereditary, reflect the well-documented pathogenetic heterogeneity for Wilms' tumors. We propose that these clones could be helpful for an improved histological characterization of Wilms' tumors.
我们采用消减杂交方法来分离在成熟肾脏和肾母细胞瘤之间差异表达的基因。我们从总的成熟肾脏互补DNA构建了一个互补DNA文库，该文库是用来自肾母细胞瘤WAGR4的过量mRNA进行消减得到的，WAGR4在11p13有生殖系缺失且在11p15有等位基因的体细胞丢失。对六个呈现差异表达模式的克隆进行了表征，这些克隆与成熟肾脏的mRNA呈阳性反应，而与肾母细胞瘤WAGR4的mRNA呈阴性反应。在这些克隆中，有两个是尚未知晓的表达序列（D11S877E和D15S109E）以及四个来自已知基因的序列：肾二肽酶（DPEP1）、αB-晶状体蛋白（CRYA2）、尿调节蛋白（UMOD）和血浆谷胱甘肽过氧化物酶（GPX2）。这些基因在11例肾母细胞瘤中的不同表达模式，无论其是否为遗传性，都反映了肾母细胞瘤已被充分证明的发病机制异质性。我们认为这些克隆可能有助于改善肾母细胞瘤的组织学特征。
