Stroeher U H, Bode L, Beutin L, Manning P A
Department of Microbiology and Immunology, University of Adelaide, Australia.
Gene. 1993 Sep 30;132(1):89-94. doi: 10.1016/0378-1119(93)90518-8.
The nucleotide sequence of the 2.1-kb EcoRI-AccI fragment of the enterohemolysin (Ehly)-associated plasmid, pEO21, has been determined. A third of this sequence encodes a 29.6-kDa protein, and coincides with the location of Tn1725 insertions which inactivate the production of Ehly. A protein of similar size (33-35 kDa) was found to be produced in large amounts by JM83[pEO21] and found to be immunologically related to the approximately 65-kDa protein made by the parental strain, C3888. DNA sequences coding for the 29.6-kDa protein of phi C3888, now designated Ehly 1, were found only in some enterohemolytic Escherichia coli indicating the existence of multiple, genetically different Ehlys.
已测定与肠溶血素（Ehly）相关的质粒pEO21的2.1 kb EcoRI - AccI片段的核苷酸序列。该序列的三分之一编码一种29.6 kDa的蛋白质，并且与Tn1725插入位点重合，这些插入会使Ehly的产生失活。发现JM83[pEO21]大量产生一种大小相似（33 - 35 kDa）的蛋白质，并且发现该蛋白质与亲本菌株C3888产生的约65 kDa蛋白质具有免疫相关性。编码phi C3888的29.6 kDa蛋白质（现命名为Ehly 1）的DNA序列仅在一些肠溶血大肠杆菌中发现，这表明存在多种遗传上不同的Ehly。
