Mendiola M V, de la Cruz F
Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
Mol Microbiol. 1989 Jul;3(7):979-84. doi: 10.1111/j.1365-2958.1989.tb00247.x.
We have determined the DNA sequences of eight different insertions of IS91 in a specifically engineered recipient plasmid of known DNA sequence (pSU300). The sequences at the termini of IS91 are 5'-CGAGTAGG...CCTATCGAT. IS91 inserts specifically 5' to either one of the tetranucleotides 5'-GAAC or 5'-CAAG, and always in the same relative orientation with respect to the sequence of the target. Except in one special case, no duplications of the recipient DNA were produced at the site of insertion.
我们已经确定了IS91在一个已知DNA序列的特定工程受体质粒（pSU300）中的八个不同插入片段的DNA序列。IS91末端的序列是5'-CGAGTAGG...CCTATCGAT。IS91特异性地插入到四核苷酸5'-GAAC或5'-CAAG之一的5'端，并且相对于靶序列总是以相同的相对方向插入。除了一种特殊情况外，在插入位点没有产生受体DNA的重复。
