Norkina O V, Kulichenko A N, Shovadaeva G A, Boshnakov R Kh, Aksenov M Iu, Popov Iu A, Tomov A Ts, K''esovskiĭ V K, Drozdov I G, Gintsburg A L
Genetika. 1993 Mar;29(3):417-22.
A 14.8 kb DNA fragment from the chromosome of Yersinia pestis TWJ was cloned and the restriction map constructed. The fragment designated as T16 and its subfragments were tested in dot-hybridization with strains of Yersinia genus and other members of Enterobacteriaceae. A species-specific DNA probe (designated MK) was constructed on the basis of the T16 fragment. As judged from restriction analysis, blot-hybridization experiments and, partially, sequencing, significant homology exists between the MK DNA probe and this one developed by Bardarov et. al. (1990). A repeated sequence in two copies was discovered in the MK fragment.
从鼠疫耶尔森氏菌TWJ染色体中克隆出一段14.8 kb的DNA片段，并构建了限制性图谱。将命名为T16的片段及其亚片段与耶尔森氏菌属菌株和肠杆菌科的其他成员进行斑点杂交试验。基于T16片段构建了一种种特异性DNA探针（命名为MK）。从限制性分析、印迹杂交实验以及部分测序判断，MK DNA探针与Bardarov等人（1990年）开发的探针之间存在显著同源性。在MK片段中发现了一个重复两次的序列。
