Bobrov A G, Filippov A A
Mol Gen Mikrobiol Virusol. 1997(2):36-40.
Cell DNAs of various species of Enterobacteriaceae were hybridized with the probes based on IS285 and IS100, mobile genetic elements of Yersinia pestis. These IS elements are found only in the genomes of all tested Y. pestis strains and a number of strains of a related bacterium Y. pseudotuberculosis. Phylogenetic relations between the tested strains and correlation of fingerprints with the geographical origin of the strains were revealed by analysis of the hybridization profiles of Y. pestis and Y. pseudotuberculosis chromosomal DNAs with IS probes. Comparison of the chromosomal IS100 profiles of Y. pestis wild strain and its nonpigmented mutant helped us determine the minimal extension of the genetic rearrangement and detect at least three copies of the IS element in the mutant region.
将基于鼠疫耶尔森氏菌的移动遗传元件IS285和IS100的探针与各种肠杆菌科细菌的细胞DNA进行杂交。这些IS元件仅在所有测试的鼠疫耶尔森氏菌菌株以及一些相关细菌假结核耶尔森氏菌菌株的基因组中发现。通过分析鼠疫耶尔森氏菌和假结核耶尔森氏菌染色体DNA与IS探针的杂交图谱，揭示了测试菌株之间的系统发育关系以及指纹图谱与菌株地理来源的相关性。比较鼠疫耶尔森氏菌野生菌株及其无色素突变体的染色体IS100图谱，有助于我们确定基因重排的最小延伸范围，并在突变区域检测到至少三个IS元件拷贝。
