双免疫扩散法、酶联免疫吸附测定法、蛋白质印迹法和CAPE印迹法检测抗SSA抗体的比较：系统性红斑狼疮中抗SSA流行情况的研究

Comparisons of double immunodiffusion, ELISA, western blot and CAPE blot for the detection of anti-SSA antibody: study of anti-SSA prevalence in systemic lupus erythematosus.

作者信息

Chretien P, Soulie E, Johanet C, Abuaf N

机构信息

Laboratoire d'Immunologie et d'Hématologie, Centre Hospitalier Intercommunal de Creteil, France.

出版信息

J Autoimmun. 1994 Jun;7(3):379-88. doi: 10.1006/jaut.1994.1027.

DOI:10.1006/jaut.1994.1027

PMID:7916909

Abstract

The specificity and sensitivity of anti-SSA (Ro) antibody detection by double immunodiffusion, ELISA, western blot and a new method named CAPE blot were studied. Using 79 normal sera, 61 sera positive for anti-SSA (Ro) antibodies (double immunodiffusion), and 39 sera without anti-SSA (Ro) antibodies but containing anti-Sm, anti-RNP, anti-SSB (La), anti-Scl 70 and anti-PCNA antibodies, we compared ELISA, western blot and CAPE blot with the immunodiffusion assay. The sensitivities of the three methods were 100, 62 and 100%, respectively. Sera from 196 patients with systemic lupus erythematosus (SLE) were tested. The prevalence of anti-SSA (Ro) antibodies in this group was scored as 26% by double immunodiffusion, 47% by ELISA, 25% by western blot and 38% by CAPE blot.

摘要

研究了采用双向免疫扩散法、酶联免疫吸附测定（ELISA）、蛋白质印迹法以及一种名为CAPE印迹的新方法检测抗SSA（Ro）抗体的特异性和敏感性。我们使用79份正常血清、61份抗SSA（Ro）抗体呈阳性的血清（双向免疫扩散法）以及39份不含抗SSA（Ro）抗体但含有抗Sm、抗RNP、抗SSB（La）、抗Scl 70和抗PCNA抗体的血清，将ELISA、蛋白质印迹法和CAPE印迹法与免疫扩散测定法进行了比较。这三种方法的敏感性分别为100%、62%和100%。对196例系统性红斑狼疮（SLE）患者的血清进行了检测。该组中抗SSA（Ro）抗体的阳性率经双向免疫扩散法测定为26%，ELISA法为47%，蛋白质印迹法为25%，CAPE印迹法为38%。