Louwagie J, Delwart E L, Mullins J I, McCutchan F E, Eddy G, Burke D S
Henry M. Jackson Foundation Research Laboratory, Rockville, Maryland 20850.
AIDS Res Hum Retroviruses. 1994 May;10(5):561-7. doi: 10.1089/aid.1994.10.561.
The spread of the human immunodeficiency virus type 1 (HIV-1) is by now virtually worldwide. An understanding of the genetic, biological, and immunological differences among isolates collected in different geographic locales is crucial for the development of globally effective vaccines. Here we report the genetic characteristics of 21 HIV-1 isolates from Brazil. The isolates were initially characterized using a heteroduplex mobility assay. The majority (17 of 21) were related to North American/European reference isolates of genetic subtype B. Four isolates belonged to a more recently identified genotype, termed subtype F. The subtype F sequences from Brazil are distinguishable in both gag and env from five other genetic subtypes of HIV-1 currently recognized. Like many locales, Brazil harbors more than one HIV-1 subtype.
1型人类免疫缺陷病毒（HIV-1）目前几乎已在全球范围内传播。了解在不同地理区域收集的病毒分离株之间的基因、生物学和免疫学差异，对于开发全球有效的疫苗至关重要。在此，我们报告来自巴西的21株HIV-1分离株的基因特征。这些分离株最初通过异源双链迁移率分析进行表征。大多数（21株中的17株）与北美/欧洲基因B亚型的参考分离株相关。四株分离株属于最近鉴定出的一种基因型，称为F亚型。来自巴西的F亚型序列在gag和env基因方面与目前公认的HIV-1其他五种基因亚型有所不同。与许多地区一样，巴西存在不止一种HIV-1亚型。
