小鼠分泌粒蛋白III的一级结构及其在缺陷小鼠中的缺失
Primary structure of mouse secretogranin III and its absence from deficient mice.
作者信息
Dopazo A, Lovenberg T W, Danielson P E, Ottiger H P, Sutcliffe J G
机构信息
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
出版信息
J Mol Neurosci. 1993 Winter;4(4):225-33. doi: 10.1007/BF02821554.
The rat brain- and pituitary-specific 1B1075 mRNA encodes a chromogranin/secretogranin-like protein, called secretogranin III (SgIII), that is a component of intracellular dense core vesicles. In order to study the function of this gene product in a mouse model system, we have isolated the murine homolog of the rat 1B1075 mRNA. This mRNA contains 2163 bp encoding a putative protein of 421 amino acids. Cleavage of the strong putative signal sequence would yield a mature protein of 51 kDa. The sequence of the encoded murine protein preserves the structural features that suggest SgIII is a member of the granin family, and allowed us to recognize and correct errors in our published rat sequence.
大鼠脑和垂体特异性的1B1075 mRNA编码一种嗜铬粒蛋白/分泌粒蛋白样蛋白，称为分泌粒蛋白III（SgIII），它是细胞内致密核心囊泡的一个组成部分。为了在小鼠模型系统中研究该基因产物的功能，我们分离了大鼠1B1075 mRNA的小鼠同源物。该mRNA含有2163个碱基对，编码一个421个氨基酸的推定蛋白。去除强推定信号序列后将产生一个51 kDa的成熟蛋白。所编码的小鼠蛋白序列保留了表明SgIII是颗粒蛋白家族成员的结构特征，并使我们能够识别和纠正我们已发表的大鼠序列中的错误。
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