Törmä H, Löntz W, Liu W, Rollman O, Vahlquist A
Department of Dermatology, Uppsala University, Akademiska Hospital, Sweden.
Br J Dermatol. 1994 Aug;131(2):243-9. doi: 10.1111/j.1365-2133.1994.tb08499.x.
Using reverse transcription coupled to polymerase chain reaction we have studied the mRNA expression of serum retinol-binding protein and cytosolic receptors for retinol and retinoic acid in skin biopsies, and in cultured epidermal keratinocytes and dermal fibroblasts. Transcripts for cellular retinol-binding protein (CRBP) I and cellular retinoic-acid-binding protein (CRABP) I were found in normal skin, keratinocytes, and fibroblasts. CRABP II transcripts were detected in skin and keratinocytes. A decreased mRNA expression of CRABP I and an increased mRNA expression of CRABP II were found in lesional psoriatic skin compared with uninvolved skin. mRNA transcripts for serum retinol-binding protein (s-RBP) were detected in all tissues and cells. The biological importance of s-RBP expression in keratinocytes and fibroblasts is not known, but hypothetically this protein may be involved in the intracellular shuttling of retinol and retinoic acid, or in the retransportation of cellular retinoids into the extracellular space.
我们运用逆转录聚合酶链反应，研究了皮肤活检组织、培养的表皮角质形成细胞和真皮成纤维细胞中血清视黄醇结合蛋白以及视黄醇和视黄酸胞质受体的mRNA表达。在正常皮肤、角质形成细胞和成纤维细胞中发现了细胞视黄醇结合蛋白（CRBP）I和细胞视黄酸结合蛋白（CRABP）I的转录本。在皮肤和角质形成细胞中检测到了CRABP II转录本。与非病变皮肤相比，在银屑病病变皮肤中发现CRABP I的mRNA表达降低，而CRABP II的mRNA表达增加。在所有组织和细胞中均检测到血清视黄醇结合蛋白（s-RBP）的mRNA转录本。s-RBP在角质形成细胞和成纤维细胞中表达的生物学意义尚不清楚，但据推测，该蛋白可能参与视黄醇和视黄酸的细胞内穿梭，或参与细胞类视黄醇向细胞外空间的再转运。
