Gates R E, Mayfield C, Allred L E
J Invest Dermatol. 1987 Jan;88(1):37-41. doi: 10.1111/1523-1747.ep12464844.
Since cellular retinol- and retinoic acid-binding proteins (CRBP and CRABP) mediate the effects of vitamin A on epidermal differentiation, the levels of these binding proteins were measured in the epidermal and dermal layers of newborn, human foreskin as well as in primary cultures of keratinocytes and fibroblasts from these layers. Ligand binding assays with saturating concentrations of all trans-[3H]retinol or of all trans-[11-3H]retinoic acid were used to quantitate amounts of binding proteins in cytosols prepared from these skin layers or cultured cells. The epidermal levels of CRABP and CRBP (60.9 +/- 14.4 and 7.3 +/- 1.7 pmol per mg cytosol protein, respectively) were markedly higher than that reported for adult epidermis but were comparable to levels in keratinocytes cultured from neonatal foreskin epidermis (61.8 +/- 7.8 and 10.7 +/- 2.5, respectively). The levels of CRABP were much lower in the foreskin dermis than in the epidermis and the levels measured in the fibroblasts cultured from this dermis were consistent with the dermal levels. However, CRBP levels in cultured dermal fibroblasts were very low and could not account for the dermal CRBP levels, suggesting that another dermal cell type has high levels of CRBP.
由于细胞视黄醇结合蛋白和视黄酸结合蛋白（CRBP和CRABP）介导维生素A对表皮分化的作用，因此对新生儿人包皮的表皮层和真皮层以及这些层的角质形成细胞和成纤维细胞原代培养物中这些结合蛋白的水平进行了测定。使用饱和浓度的全反式-[3H]视黄醇或全反式-[11-3H]视黄酸进行配体结合测定，以定量从这些皮肤层或培养细胞制备的细胞溶质中结合蛋白的量。CRABP和CRBP的表皮水平（分别为每毫克细胞溶质蛋白60.9±14.4和7.3±1.7 pmol）明显高于报道的成人表皮水平，但与新生儿包皮表皮培养的角质形成细胞中的水平相当（分别为61.8±7.8和10.7±2.5）。包皮真皮中CRABP的水平远低于表皮，并且从该真皮培养的成纤维细胞中测得的水平与真皮水平一致。然而，培养的真皮成纤维细胞中CRBP的水平非常低，无法解释真皮中CRBP的水平，这表明另一种真皮细胞类型具有高水平的CRBP。
