Joore J, van der Lans G B, Lanser P H, Vervaart J M, Zivkovic D, Speksnijder J E, Kruijer W
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Mech Dev. 1994 May;46(2):137-50. doi: 10.1016/0925-4773(94)90082-5.
The cDNAs encoding the zebrafish homologs of retinoic acid receptor alpha(zRAR alpha) and gamma (zRAR gamma) were isolated and their expression studied in normal and retinoic acid (RA) treated embryos. Expression boundaries in the central nervous system are clearly different from those observed in the mouse, which can only partly be explained by morphogenetic differences. Treatment of embryos with RA induces ectopic zRAR gamma expression in anterior brain structures and both zRAR alpha and zRAR gamma expression in the eyes. Furthermore, striking differences occur in the zRAR gamma expression pattern in pharyngeal arch mesenchyme. Since the development of all of these structures has been shown to be affected by exogenous RA, our data suggest a role for zRAR alpha and zRAR gamma in the establishment of the RA phenotype in zebrafish.
分离出编码斑马鱼视黄酸受体α（zRARα）和γ（zRARγ）同源物的cDNA，并研究它们在正常胚胎和视黄酸（RA）处理胚胎中的表达。中枢神经系统中的表达边界与在小鼠中观察到的明显不同，这只能部分地由形态发生差异来解释。用RA处理胚胎会在前脑结构中诱导异位zRARγ表达，并在眼睛中诱导zRARα和zRARγ表达。此外，咽弓间充质中的zRARγ表达模式存在显著差异。由于所有这些结构的发育已被证明受外源性RA影响，我们的数据表明zRARα和zRARγ在斑马鱼RA表型的建立中起作用。
