Hirata S, Mouri-Yamada N, Kato J
Department of Obstetrics and Gynecology, Yamanashi Medical University, Japan.
J Steroid Biochem Mol Biol. 1994 Sep;50(5-6):293-7. doi: 10.1016/0960-0760(94)90134-1.
The effects of neonatal hypothyroidism on the gene expression of progesterone receptors (PRs) in the 8-day-old female rat brain were examined. The levels of PR ('A'+'B')-, PR ('B')-, estrogen receptor (ER)- and beta-actin mRNAs in the cerebral cortex and hypothalamus-preoptic area of propylthiouracil-treated rats (PTU group) or untreated rats (control group) were analyzed using a quantitative reverse transcription-polymerase chain reaction-Southern blotting assay. When the levels of PR mRNAs were calibrated by respective levels of beta-actin mRNA, the levels of PR ('A'+'B')- and PR ('B') mRNAs in the cerebral cortex of the PTU group were markedly less than those in the control group with no significant changes in the levels of PR messages in the hypothalamus-preoptic area of both groups. No significant difference in the calibrated levels of ER mRNA between both groups was found in these tissues. These results on the PR-, and ER mRNAs were essentially similar to those on the levels of PR- and ER proteins previously reported suggesting that thyroid hormones affect the transcriptional machinery of PR in the developing brain, in a region-specific manner.
研究了新生期甲状腺功能减退对8日龄雌性大鼠大脑中孕激素受体（PRs）基因表达的影响。采用定量逆转录-聚合酶链反应- Southern印迹分析法，分析丙硫氧嘧啶处理大鼠（PTU组）或未处理大鼠（对照组）大脑皮层和下丘脑-视前区中PR（“A”+“B”）、PR（“B”）、雌激素受体（ER）和β-肌动蛋白mRNA的水平。当用各自的β-肌动蛋白mRNA水平校准PR mRNA水平时，PTU组大脑皮层中PR（“A”+“B”）和PR（“B”）mRNA的水平明显低于对照组，而两组下丘脑-视前区PR信息水平无显著变化。在这些组织中，两组之间ER mRNA的校准水平没有显著差异。这些关于PR和ER mRNA的结果与先前报道的PR和ER蛋白水平的结果基本相似，表明甲状腺激素以区域特异性方式影响发育中大脑中PR的转录机制。
