Hatamochi A, Arakawa M, Takeda K, Ueki H
Department of Dermatology, Kawasaki Medical School, Okayama, Japan.
J Dermatol Sci. 1994 Jun;7(3):210-6. doi: 10.1016/0923-1811(94)90097-3.
The effects of Werner's syndrome (WS) fibroblast-conditioned medium on cell proliferation and collagen production of normal fibroblasts were studied using four WS fibroblast strains. The conditioned medium from the WS fibroblasts brought about activation of normal fibroblast proliferation, whereas, that from late passage normal fibroblasts and fibroblasts from aged donors' skin did not. Collagen and non-collagenous protein synthesis in normal fibroblasts were increased by the addition of conditioned medium from the WS fibroblasts, but the relative rates of collagen synthesis and non-collagenous protein synthesis were unaltered. These results suggest that activation of fibroblast proliferation by conditioned medium is one of the characteristics of WS fibroblasts.
使用四株沃纳综合征（WS）成纤维细胞系，研究了WS成纤维细胞条件培养基对正常成纤维细胞增殖和胶原蛋白产生的影响。WS成纤维细胞的条件培养基可激活正常成纤维细胞的增殖，而来自传代后期正常成纤维细胞和老年供体皮肤成纤维细胞的条件培养基则不能。添加WS成纤维细胞的条件培养基可增加正常成纤维细胞中胶原蛋白和非胶原蛋白的合成，但胶原蛋白合成与非胶原蛋白合成的相对速率未改变。这些结果表明，条件培养基激活成纤维细胞增殖是WS成纤维细胞的特征之一。
