Strauss K I, Isaacs K R, Ha Q N, Jacobowitz D M
NIMH, Laboratory of Clinical Science, Bethesda, MD 20892.
Biochim Biophys Acta. 1994 Oct 18;1219(2):435-40. doi: 10.1016/0167-4781(94)90069-8.
Calretinin, a highly evolutionarily conserved E-F hand calcium binding protein, is expressed predominantly in neurons, with a few exceptions. The function of calretinin is not known. We demonstrate the expression of calretinin mRNA and protein in rat testes. Immunocytochemistry and in situ hybridization reveal that calretinin expression in testis is localized to the interstitial Leydig cells. Western blot and ribonuclease protection analyses show that calretinin protein and mRNA in testis is the same as that expressed in brain. It is suggested that calretinin may play a role in the production of testosterone.
钙视网膜蛋白是一种在进化上高度保守的E-F手型钙结合蛋白，主要在神经元中表达，仅有少数例外情况。钙视网膜蛋白的功能尚不清楚。我们证明了大鼠睾丸中钙视网膜蛋白mRNA和蛋白的表达。免疫细胞化学和原位杂交显示，睾丸中钙视网膜蛋白的表达定位于间质中的莱迪希细胞。蛋白质免疫印迹和核糖核酸酶保护分析表明，睾丸中的钙视网膜蛋白和mRNA与大脑中表达的相同。提示钙视网膜蛋白可能在睾酮的产生中发挥作用。
